《植物生理学报》 2018, 54(8): 1333-1340

水稻温度超敏突变体ths1的鉴定和基因定位

徐纪明, 姜小燕, 赵鹏, 于亚男, 毛传澡, 吴忠长^*

浙江大学生命科学学院, 杭州310058

通信作者：吴忠长；E-mail: wzchang@zju.edu.cn

摘　要：

从籼稻品种‘Kasalath’甲基磺酸乙酯(ethyl methanesulfonate, EMS)诱变突变体库中筛选到一个温度敏感突变体ths1 (temperature hyper-sensitive 1)。与野生型相比, 突变体ths1的主根伸长和侧根发育对温度变化非常敏感: 在30°C条件下ths1表型与野生型一致; 在温度低于26°C时, ths1主根变短, 侧根缺失。在低温(24°C)条件下ths1侧根原基停留在原基起始阶段, 根尖ROS含量显著高于野生型。遗传分析显示, ths1表型由半显性单基因控制。利用F₂群体对ths1基因进行定位, 将突变基因定位在第1染色体长臂着丝粒附近C01M14到RM11110两个标记之间的694 kb区间。研究结果为进一步克隆THS1基因, 揭示植物感受外界温度变化的分子生理机制奠定基础。

关键词：水稻; 温度敏感; 侧根; 基因定位

收稿：2018-06-11   修定：2018-07-12

资助：国家自然科学基金(31701983和31770281)。

Characterization and gene mapping of a temperature hyper-sensitive mutant ths1 in rice

XU Ji-Ming, JIANG Xiao-Yan, ZHAO Peng, YU Ya-Nan, MAO Chuan-Zao, WU Zhong-Chang^*

College of Life Science, Zhejiang University, Hangzhou 310058, China

Corresponding author: WU Zhong-Chang; E-mail: wzchang@zju.edu.cn

Abstract:

A temperature hyper-sensitive mutant ths1 (temperature hyper-sensitive 1) was isolated from a rice EMS (ethyl methanesulfonate, EMS)-generated mutant library (‘Kasalath’, indica). The development of primary root and lateral roots of ths1 mutant showed hyper-sensitive to temperature changes. The phenotype of ths1 mutant was consistent with wild-type at 30°C, but under the condition of temperature below 26°C, the primary root of ths1 mutant was shorter and the lateral roots growth was inhibited compared with the wide type. The development of lateral roots of the mutant was inhibited at the initial stage of primordium, and the ROS content in root tip of ths1 mutant was significantly higher than wild type when cultured at low temperature (24°C). Genetic analysis showed that ths1 possessed a semi-dominant mutation in a single nuclear locus. F₂ population was constructed by crossing the mutant with ‘Nipponbare’, and the mutants from extreme individuals were selected to map gene ths1. The gene was located in the 694 kb region near the centromere between the molecular markers C01M14 and RM11110 on the long arm of chromosome 1. The results of this study lay a foundation for further cloning THS1 gene and revealing the molecular mechanism of plant perception and response to temperature.

Key words: rice; temperature sensitive; lateral root; gene mapping